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Description
Mouse BCL2A1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Bcl2-related protein A1 (BCL2A1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Bcl2-related protein A1 (BCL2A1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Bcl2 Related Protein A1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Bcl2-related protein A1 (BCL2A1) is a protein encoded by the BCL2A1 gene. This gene encodes a member of the Bcl2 protein family. Proteins in this family form heterodimers or homodimers and act as anti-apoptotic and pro-apoptotic regulators involved in various cellular activities, such as embryonic development, homeostasis, and tumorigenesis. The protein encoded by this gene can reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
Based on 15 reviews
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Product Reviews
★★★★★ 5
My favorite desk item
Color: Wood
I love my coffee but it must be hot-every sip! I'd get up at work so often to go reheat my coffee in the nuker & don'tlike the microwave AND a hater coworker even commented to me that she noticed "how much I get up from my desk" so I thought, they gotta be making coffee hot plates for desktops these days & boom, they do!! I like the wood finish, right my aesthetic alley. The plate has 3 settings; high, medium & low. Even has an auto shutoff after..idk how many hours so built in safety. I've even put my ceramic cup of soup on there to keep it warm while I work through lunch a couple times & it worked for my soup on the high setting. Coffee goes to medium when I first get in since it cools on the car ride over & then down to low once it's hot again. Will be getting one for the home office here soon.
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Reviewed in the United States on May 2, 2026
★★★★★ 5
Mug warmer
Color: Wood
This is a well made mug warmer, heats up quickly and convenient to use. The heat ratio is high making it easy to select the level of heat one wants to have to enjoy their coffee, tea, hot chocolate, etc. The only default I have with this product is how to turn on the warmer. It's convenient but if something is sitting next to the warmer, the warmer turns on too easily. I would recommend the manufacturer use some type of switch to turn on, adjust and turn off the warmer due to the chances of heating something up that was not intentional!
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Reviewed in the United States on April 26, 2026
★★★★★ 5
Simple and very useful
Color: White
Right out of the box it works just as expected, gets perfectly hot. Even with a small cup, my coffee typically cooks to quickly for my taste. Jumping up to give it a pop in the microwave gets old fast. Problem solved. For already hot coffee, the low is enough to keep it sippable. I'm pretty sure I could hear a can of soup from room temp on the high setting. I do hope it is doable because this is a perfect hot plate.
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Reviewed in the United States on April 22, 2026
★★★★★ 4
Works great but design flaw.
Color: Wood
Works great. 3 settings. The first is HOT. I usually use the middle or low setting and they keep my cup warm and drinkable. There is a flaw though with this device. The warming button is so easy to touch /come on that several times on my desk, it has come on without me realizing it from paper hitting it, a pen rolled against it or simply moving it over and it is on highest setting when it comes on so I have burned my fingers now twice. I just always double and triple check it isn’t on.
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Reviewed in the United States on February 14, 2026
★★★★★ 5
Powerful Mug Warmer with Excellent Heat Output and Large Heating Surface
Color: Wood, Color: Wood
CAUTION: Unplug when not using. It is very easy to activate or turn on by mistake. Simply brushing up next to it will turn it on.
Nonetheless, I use this SWEETFULL coffee mug warmer every morning and afternoon. It’s usually one of the first things I turn on so I can preheat my latte cup before pulling espresso. I like my lattes very hot, and preheating the cup helps prevent heat loss during extraction. I also use it as a candle warmer as an alternative to open flame, which I prefer for indoor air quality.
The heat output is impressive. Even on the lowest setting, it gets very hot and reaches usable temperature quickly. The large heating surface is a major advantage. My latte and cappuccino cups have a wide mouth, and this warmer heats the entire base evenly rather than just the center, which makes a noticeable difference in temperature retention.
Warm-up time is reasonable, though slightly faster heating would make it even better, especially during busy mornings when I’m trying to get out the door. That said, it’s not slow enough to be a real drawback, and once it’s hot, it performs consistently.
Overall, this is a strong performer that does exactly what it’s designed to do. It’s a great option for coffee lovers who want hotter drinks for longer, and for candle users who prefer a flame-free, cleaner alternative inside the home.
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Reviewed in the United States on February 22, 2026
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